Structural, metabolic and behavioral relationship between a regenerative ciliate and its endosymbiotic algae

Using a wild species of the large and regenerative unicellular ciliate, Stentor, we are studying the structural, metabolic and behavioural relationship between the ciliate and its algal endosymbionts. Given that this organism is native to a pond in Woods Hole, MA, this project is being pursued in the context of the Marine Biological Laboratory's Physiology Course, an intensive summer research course for graduate students and post-doctoral fellows.

How nuclei expand to form functional organelles

Through a highly collaborative effort between our lab and the labs of Jay Gatlin, Jun Allard and Hernan Garcia, we used quantitative live-cell microscopy assays, mathematical modelling and several model systems to determine how the nucleus is assembled into a functional organelle. Using our model to predict nuclear assembly regulators, we uncovered a role for nucleo-cytoplasmic trafficking in modulating the amount of factors regulating nuclear surface area and hence, nuclear surface tension.

Associated preprint

Boudreau V., Hazel J., Sellinger J.K., Chen P., Manakova K., Radzyminski R., Garcia H.G., Allard J., Gatlin J., Maddox P.S. (2018) Nucleo-cytoplasmic trafficking regulates nuclear surface area during nuclear organogenesis. bioRxiv 326140; doi: https://doi.org/10.1101/326140.

Regulation of centrosome separation during mitotic spindle formation

Having identified a functional interaction between a cell cycle phosphatase and a nuclear lamina component, we investigated the role for these components in regulating early mitotic events in the developing worm embryo. We uncovered a role for the phosphatase and the nuclear lamina in regulating centrosome separation and positioning during mitotic spindle formation of the first embryonic mitosis.

Publication

Boudreau V., Chen R., Edwards A., Muhammad S., Maddox P.S. (2019) PP2A-B55/SUR-6 collaborates with the nuclear lamina for centrosome separation during mitotic entry. Mol Biol Cell; doi: 10.1091/mbc.E18-10-0631. Featured in MBoC's Fifth Annual Special Issue on Quantitative Cell Biology.

Publications

Research articles

Dumont M.*, Gamba R.*, Gestraud P, Klaasen S, Worrall J.T., De Vries S.G., Boudreau V., Maddox P.S., Lens S.M.A., Kops G.J.P.L., Mc Clelland S., Miga K.H. & Fachinetti D. (2019) Human chromosome-specific aneuploidy is influenced by DNA-dependent centromeric features. EMBO J; doi: 10.15252/embj.2019102924 *equal contribution

Boudreau V., Chen R., Edwards A., Muhammad S., Maddox P.S. (2019) PP2A-B55/SUR-6 collaborates with the nuclear lamina for centrosome separation during mitotic entry. Mol Biol Cell; doi: 10.1091/mbc.E18-10-0631. Featured in MBoC's Fifth Annual Special Issue on Quantitative Cell Biology.

Hatkevich T., Boudreau V., Rubin T., Huynh J.-R., Maddox P.S., Sekelsky J. (2019) Centromeric SMC1 promotes centromere clustering and stabilizes meiotic homolog pairing. PLoS Genetics; doi: 10.1371/journal.pgen.1008412.

Mehsen M., Boudreau V., Garrido D., Bourouh M., Larouche M., Maddox P.S., Swan A., Archambault V. (2018) PP2A-B55 promotes nuclear envelope reformation after mitosis in Drosophila. J Cell Biology; doi: 10.1083/jcb.201804018.

Byrnes A.E., Lowe B.F., Boudreau V., Slep K.C. Polarized TOG arrays cooperatively bind tubulin to promote microtubule dynamics. (in revision)

Boudreau V., Hazel J., Sellinger J.K., Chen P., Manakova K., Radzyminski R., Garcia H.G., Allard J., Gatlin J., Maddox P.S. (2018) Nucleo-cytoplasmic trafficking regulates nuclear surface area during nuclear organogenesis. bioRxiv 326140; doi: 10.1101/326140.

Science policy with the Future of Research (FoR)

Bankston, A., Davis, S. M., Moore, E., Niziolek, C. A., Boudreau V.. (2020) Research Culture: Why scientific societies should involve more early-career researchers. eLife; doi: 10.7554/eLife.60829.

Methods/protocols/chapters

Ryan J., Gerhold A.R., Boudreau V., Smith L., Maddox P.S. (2017) Introduction to Modern Methods in Light Microscopy. In: Markaki Y., Harz H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY; doi: 10.1007/978-1-4939-6810-7_1.

Other

Interview with the Ackland Art Museum (UNC-Chapel Hill) for The Beautiful Brain: The Drawings of Santiago Ramón y Cajal exhibit.

Phone

(919) 903-7212

Address

University of California, San Francisco and Berkeley
Berkeley, CA
United States of America